EZ Yeast ColonyScreen Kit is an easy and fast method for genotyping,
strain verification, yeast two-hybrid screening, gene amplification,
etc., directly from yeast colonies without the
steps of zymolyase digestion of yeast cell wall, and preparation of yeast
genomic DNA. It also provides a
rapid method for PCR yeast genes directly from yeast colonies without making
and fast for genotyping, strain verification, yeast two-hybrid screening, gene
directly from yeast colonies.
need to grow overnight culture for plasmid or genomic DNA preparation.
the use of any restriction enzyme for analysis when amplified by 2x
Taq Mix without dye.
DNA Taq polymerase and dNTPs with red dye. You
just need to add primers.
to load on the gel without adding loading dye when using 2x
Taq Mix Red.
of the kit:
Taq Mix Red or 2x Taq Mix (without dye): 1 ml
lysis solution: 1 ml
kit is sufficient for reactions with 100 colonies.