EZ E. coli ColonyScreen Kit is an easy
and fast method to screen positive clones directly from plate for gene cloning.
It also provides a rapid method for PCR E.
coli genes directly from colonies without making genomic DNA.
and fast to screen positive clones of gene cloning directly from E.
way for PCR E. coli genes directly
need to grow overnight culture for plasmid minipreps and genomic DNA
the use of any restriction enzyme for analysis when amplified by 2x
Taq Mix without dye.
DNA Taq polymerase and dNTPs with red dye. You
just need to add primers.
to load on the gel without adding loading dye.
of the kit:
Taq Mix Red or 2x Taq Mix (without dye): 1 ml
lysis solution: 1 ml
kit is sufficient for screening 100 colonies.