Our
ultra-pure dNTPs are certified free of nucleases and phosphatases. The
functional purity is tested in Sanger dideoxy sequencing reactions. It
is also tested by filling-in of 5′ overhangs and primer extensions
using Klenow and several thermostable DNA polymerases. The chemical
purity of this preparation is determined by HPLC, requiring a minimum
passing specification of 99% triphosphate. Nucleotide concentrations are
determined by measurements of absorbance. Each nucleotide is supplied at
100mM/40µmol in sterile, doubly distilled water. Dilutions of these
nucleotides can be made using sterile, doubly distilled water.
